以刺巴西甲螨作为受试生物,辛硫磷为污染物,采用两个辛硫磷处理时间(48 h和96 h),测定六种酶(乙酰胆碱酯酶、腺三磷酶、谷胱甘肽硫转移酶、过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶)的活性随辛硫磷浓度变化的趋势.结果显示了刺巴西甲螨体内酶活性与辛硫磷浓度间存在一定的剂量-效应关系,可为生物指示土壤辛硫磷农药污染提供参考依据.其中乙酰胆碱酯酶和腺三磷酶随辛硫磷浓度增加及处理时间延长表现为抑制效应,而谷胱甘肽硫转移酶、过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶的活性总体表现为诱导效应.%Brasilobates spinosus Fujita collected from pollution-free soil by Tullgern method was used as subject in an experiment to explore effect of phoxim on activities of enzymes (acetylcholinesterase, adenosinetriphos-phatase, glutathione-S-transferase, glutathione peroxidase, superoxide dismutase and catalase) in B. spinosus Fujita, 48 h and 96 h after treatment with phoxim. Results indicate that the activities of acetylcholinesterase and adeno-sinetriphosphatase declined obviously with increasing phoxim concentration and elapsing time, while the activities of the other four enzymes showed a reverse trend. A certain dose-effect relationship was found to be in existence between activities of the enzymes and concentration of phoxim, and the findings may serve as reference or basis for use of bio-indicator of phoxim pollution of soils.
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