新疆地区扇头蜱中Candidatus Rickettsia barbariae的检测与序列分析

摘要

To investigate the prevalence of Candidatus Rickettsia barbariae in ticks from Xinjiang,total of 90 Rhipicephalus sanguineus and 420 Rhipicephalus turanicus were collected from Yuli area and the genes of ompA,ompB,gltA and 17kDa of Candidatus Rickettsia barbariae were detect with PCR.The target genes of ompA,ompB,gltA and 17kDa of Candidatus Rickettsia barbariae were detected from 10 Rh.sanguineus and 53 Rh.turanicus with rates as 11.11％ and 12.62％ respectively.The sequences obtained from these ticks were identical to the corresponding sequence in the positive samples.By BLAST analysis,the ompA,ompB partial sequences were 99.8％ (583/584) and 100％ (768/768) identical to the corresponding sequence of Candidatus Rickettsia barbariae strain from Rh.turanicus in Italy (EU272186.1,EU272187.1) respectively.The gltA and 17kDa partial sequence were also 100％ identical (375/375,356/356) to the corresponding sequence of Candidatus Rickettsia barbariae strain from Vermipsylla alakurt in Xinjiang (KT284716.1,KT284715.1).The manifestations of Candidatus Rickettsia barbariae occur in Rh.sanguineus and Rh.turanicus might suggest the potential health threat of the rickettsia organism to residents in Xinjiang area.%为了解新疆地区血红扇头蜱和图兰扇头蜱中Candidatus Rickettsia barbariae立克次体感染状况,从新疆尉犁县采集蜱标本510只,其中血红扇头蜱90只,图兰扇头蜱420只.利用PCR方法对C.R.barbariae 立克次体ompA、ompB、gltA和17kDa基因片段进行扩增.10只血红扇头蜱和53只图兰扇头蜱检测出C.R.barbariae阳性,阳性率分别为11.11％和12.62％.经序列分析,同一目的基因阳性序列相同,为同一序列.经比对分析,ompA基因序列与意大利撒丁岛的图兰扇头蜱中检测出的C.R.barbariae序列(EU272186.1)同源性为99.8％ (583/584).所测得ompB基因序列与意大利撒丁岛图兰扇头蜱中检测出的的C.R.barbariae OmpB基因序列(EU272187.1)同源性达100％ (768/768).所测得的gltA基因序列、17kDa基因序列与新疆花蠕形蚤Vermipsylla alakurt检测出的相应基因序列(KT284716.1、KT284715.1)同源性达100％ (375/375、356/356).结果表明,新疆尉犁县地区血红扇头蜱和图兰扇头蜱中存在C.R.barbariae自然感染,感染率较高.