大连渤海老虎滩海域沉积物可培养放线菌的多样性

摘要

[目的]研究大连渤海老虎滩海域可培养放线菌的多样性.[方法]利用5种不同的培养基分离、培养海洋沉积物中的放线菌,并用16s rRNA基因序列对部分放线菌株进行系统发育分析.[结果]根据菌落表型共分离到1215株放线菌.选择271株具有代表性的菌株进行16S rRNA分析,结果表明,251株(92.26%)属于放线菌门,覆盖11个科,15个属;其余20株属于厚壁门和变形菌门;有7株为潜在的新种.[结论]大连渤海老虎滩海域的沉积物中存在较为丰富的放线菌和新种资源,这些菌株为将来开发新的微生物代谢产物奠定了基础.%[Objective]To study the diversity of culturable actinomycetes isolated from the sea deposit of Tiger beach at Bohai bay, Dalian, China.[Methods]By using five different media strains we isolated actinomycetes strains from the sea deposit of Tiger beach at Bohai bay, Dalian, China.Partial 16S rRNA sequences were carried out to characterize the diversity of culturable actinomycetes.[Results]A total of 1215 colonies with phenotypical actinomycetes were isolated, of which 271 were classified by 16S rRNA phylogenetic analysis.The data showed that 251 strains were homologous with Actinobacteria (92.26％ ) including 15 genera of 11 families,and the remaining 20 strains were possible members of the phylum Firmicutes and Proteobacteria.There were 7 strains (3-166,3-117,3-435,5-186-2,5-9,5-171,3-134) to be preliminarily identified as unreported species.[Conclusion]A high diversity of culturable actinomycets both in terms of the number of species and phylogenetic composition was presented from the sea deposit of Tiger beach in Bohai bay.