南方红豆杉根际溶无机磷细菌的筛选、鉴定及其促生效果

摘要

[目的]对南方红豆杉(Taxus chinensis var.mairei)根际溶无机磷细菌进行了分离、筛选与鉴定,并对获得的高效溶磷菌株进行了温室盆栽试验.本研究为通过生物途径改善南方红豆杉磷素供应,促进其生长提供了优良的菌株资源.[方法]利用选择培养基从南方红豆杉根际土壤中共分离出具溶磷能力的细菌；采用NBRI-BPB培养基进行复筛获得溶磷能力较强的溶无机磷细菌；并采用钼锑抗比色法测量其在NBRIP培养基中经4d发酵后的可溶性磷含量；通过形态指标、生理生化测定、Biolog系统和16S rDNA序列分析鉴定细菌种类；并进行了溶磷菌株的室内盆栽实生苗接种试验.[结论]从南方红豆杉根际共分离出4株高效溶磷细菌,分别鉴定为荧光假单胞菌(Pseudomonas fluorescens)、蜡状芽胞杆菌(Bacillus cereus)、草木樨中华根瘤菌(Sinorhizobium meliloti)和地衣芽胞杆菌(Bacillus licheniformis)；4株细菌对南方红豆杉苗期的生长有明显的促进作用.%[ Objective] Phosphate-solubilizing bacteria (PSB) were isolated, screened and identified from the rhizosphere of Taxus chinensis var. Mairei, and growth-promoting effects on T. Chinensis var. Mairei by high effective PSB were determined. [Methods] By using selective culture media, PSB were isolated from rhizospheric soil, the high effective PSB was further screened using NBRI-BPB medium, and the molybdenum-antimony anti-spectrophotometric method was applied to determine the phosphate-dissolving ability of the high effective PSB after four days fermentation in NBRIP medium. Bacteria were identified by the Biolog system combined with 16S rDNA gene sequence analysis and morphological, physiological and biochemical characteristics. The inoculation test in potted seedlings was carried out under the greenhouse. [ Conclusion ] Four strains of high effective PSB were screened and identified as Pseudomonas fluorescent, Bacillus cereus, Sinorhizobium meliloti and Bacillus licheniformis, respectively. These strains had significant effects on improving the growth of the seedlings of T. Chinensis var. Mairei.