逆流萃取—反相高效液相色谱法测定川芎中川芎嗪的含量

摘要

An analytical method for determination of tetramethylpyrazine (TMP) in ligusticum chuanxiong hort by RP-HPLC coupled with countercurrent extraction used for the preconcentration of trace TMP was established. The column was packed with Zorbax ODS (250 mm×4 mm，5 μm). The mobile phase consisted of methanol water (1 % acetic acid)(35∶65)，the flow rate was 0.8 ml/min and detection was performed at 300 nm. The calibration curves showed good linearity over the range of 0.41 μg/ml to 4.1 μg/ml，γ＝0.9993，the recoveries were 92.3 %. The method was simple，fast，sensitive and accurate and could be applied to the analysis of ligusticum chuanxiong hort. The results showed that the content of TMP in ligusticum chuanxionghort were different with different sources.%采用逆流萃取与反相高效液相色谱相结合的方法，富集并测定了川芎中川芎嗪的含量。色谱柱：Zorbax ODS柱，流动相：甲醇：水(1%醋酸)=35：65，流速：0.8 ml/min，检测波长：300 nm，线性范围：0.41 μg/ml～4.1 μg/ml，r＝0.9993，回收率：92.3%。方法简便、快速、灵敏。用此法对购自不同地区的川芎进行了分析，结果提示：市售川芎的川芎嗪含量差异较大。