磷脂酶A2抑制剂对肝脏缺血再灌注的保护作用

摘要

To study the relationship between the disturbance of nitric oxide/endothelin (NO/ET) and the hepatic ischemia/reperfusion (I/R) injury as well as the regulation of NO/ET by the phospholipase A2 inhibitor indomethacin (INDO)，the changes of NO/ET and their relationship with the hepatic I/R injury were compared between the I/R group and the INDO group in a hepatic I/R model. In the acute phase of hepatic reperfusion，the ratio of NO/ET was reduced，which was contributed to the significant reduction of NO2-/NO3- (the metabolic product　of NO) but significant elevation of ET in the blood. TNF-α in the blood，hepatic MDA and the leakage of enzymes were all increased but the hepatic ATP was reduced，the hepatic damage was deteriorated. INDO could improve the balance relationship of NO/ET by preventing the reduction of NO2-/NO3- and alleviate the injury of the liver. It was considered that the hepatic I/R injury might be concerned with the disturbance of NO/ET，the protection of INDO against hepatic I/R in the acute phase might be relative to its regulation on NO/ET.%为研究一氧化氮/内皮素（NO/ET）失衡与肝缺血再灌注（I/R）损伤的关系以及磷脂酶A2抑制剂消炎痛(INDO)对NO/ET的调节作用，采用肝I/R模型，比较I/R组和INDO组NO/ET的变化情况及其与肝I/R损伤的关系。再灌注急性期血NO代谢产物（NO2－/NO3－）降低、ET升高致NO/ET比值降低，血TNF-α、肝丙二醛（MDA）及酶的漏出增加，肝ATP含量降低，肝损伤加重；INDO可阻止再灌注后NO2－/NO3－下降、改善NO/ET平衡、减轻肝损伤。认为肝I/R损伤与NO/ET失衡有关，INDO对肝I/R急性期的保护作用与其对NO/ET的调节作用有关。