8种人恶性淋巴瘤细胞系P16基因突变的研究

摘要

In order to determine the relationship between P16 and the tumorigenesis of malignant lymphoma，silver-stained PCR-SSCP analysis and direct sequencing were used to evaluate the alterations of P16 gene in 8 human malignant lymphoma cell lines. A homolygous loss was found in SU-DHL-9 while mutations of P16 exon 2 in SU-DHL-1 and Daudi. Sequencing of the PCR-product showed three point mutations in SU-DHL-1，which were GAC to AAC in codon 72，GOT to ACT in codon 77，and a insertion of C between codon 82 and 83，respectively. This study suggested the mutations of P16 gene are highly frequent in B-cell lymphoma cell lines and may play an important role in its tumorigenesis.%为确定P16基因与恶性淋巴瘤发生发展的关系，对8种人恶性淋巴瘤细胞系中P16基因突变情况进行研究。用银染PCR-SSCP方法对P16基因第二外显子突变情况进行研究，并对检出突变者用自动测序仪进行测序分析。检出1例纯合缺失，2例P16基因第二外显子突变。测序分析发现SU-DHL-1 P16基因第二外显子有3处点突变，即密码子72的GAC突变为AAC，密码子77的GCT突变为ACT,密码子82与83之间插入了一个C。认为 P16基因在B细胞恶性淋巴瘤细胞系中的突变率较高，提示其改变可能参与恶性淋巴瘤的发生发展。