目的 探讨转录因子c-Ets1对K562细胞BCR-ABL融合基因表达的影响.方法 将c-Ets1基因转染K562细胞,RT-PCR检测转染后BCR-ABL融合基因mRNA的表达;Western blot检测转染后融合蛋白p210的表达;MTT法检测对细胞增殖的影响;生物信息学方法初步分析BCR-ABL启动子的功能.结果 RT-PCR检测表明,c-Ets1可以抑制BCR-ABL基因mRNA的表达;Western blot检测显示c-Ets1可以抑制p210蛋白的表达;转染c-Ets1后细胞的生长抑制率为(25.52±6.75)%;BCR-ABL启动子区存在c-Ets1的潜在作用靶点.结论 c-Ets1可以抑制K562细胞BCR-ABL基因及p210的表达,进而抑制细胞的增殖.%Objective To explore the effects of transcription factor c Etsl on the BCR ABL fusion gene expression in K562 cells- Methods After the c Etsl gene was transfected into K562 cells, the expression of BCR ABL gene was detected by RT PCR and Western blot respectively,the proliferation of K562 cells was measured by MTT methods ,and the BCR ABL promoter region was analyzed by bioinformatics- Results RT PCR revealed that c Etsl could inhibit the expression of BCR ABL both at mRNA and protein levels- The growth inhibition rate of K562 cells by c Etsl was(25. 52+6. 75) %. Potential targets of c Ets1 were found in the promoter region of BCR ABL. Conclusion c Etsl can down regulate the expression of BCR ABL,which may account for its mechanism in inhibiting the proliferation of K562 cells.
展开▼
