牙周膜成纤维细胞与静电纺丝纳米纤维的生物相容性研究

摘要

目的 研究不同比例的静电纺丝聚乳酸/聚己内酯(PLA/PCL)纳米纤维与牙周膜成纤维细胞(PDLF)的生物相容性,探索其作为牙周膜组织工程支架的可行性.方法 聚乳酸(PLA)和聚己内酯(PCL)分别按重量比75:25、50:50和25:75制成混合溶液,应用静电纺丝技术制成3种比例的纳米纤维支架.将PDLF接种于3种比例的支架上,经细胞培养,通过光学显微镜观察PDLF在3种支架上的形态;MTT法检测PDLF在支架上的增殖,荧光显微镜下计数细胞,检测粘附能力;活死细胞染色法比较PDLF在3种比例支架上的活力.结果 在体外培养条件下,PDLF在3种比例支架上均能生长,其中50:50支架上的PDLF增殖曲线最接近常规培养细胞的曲线,明显较其他2种支架上PDLF数量多;50:50支架对PDLF的粘附作用明显强于另外2种;50:50支架对PDLF活性的影响最小,优于其他比例的支架.结论 PDLF能在不同比例的PLA/PCL纳米纤维上生长,其中,按重量比为50:50合成的支架在与牙周膜成纤维细胞共培养时体现出较好的生物相容性,可作为牙周膜组织工程候选支架材料.%Objective To explore the cellular biocompatibility of electrospun PLA/PCL blending nanofibrous scaffolds in different composite ratios with periodontal ligament fibroblasts(PDLF). Methods PLA and PCL were blended in the weight composite ratios of 75 : 25 ,50 : 50 and 25 : 75. The nanofibrous scaffolds of such three blends were synthesized by the electro spinning technique and morphologically detected under the optical microscopy and scanning electron microscopy. PDLF were seeded and cultured on the nanofibrous scaffolds to evaluate the morphology of the synthesis from three different ratios. MTT assay was used to investigate the proliferation and attachment of PDLF on nanofibrous scaffolds. The viability of PDLF was ex amined by living and dead cell staining. Results In vitro , PDLF can grow on the fibers synthesized from three ratios. The growth curves of PDLF on the nanofibrous scaffolds of 50 : 50 weight ratio was not significantly different from that of the con ventional cultured cells. Moreover,PDLF in 50 : 50 group demonstrated a greater number,faster proliferation and more signifi cant attachment and viability than other two groups. Conclusion The electrospun nanofibrous scaffolds of 50 : 50 behaved bet ter than 75 : 25 and 25 : 75 scaffolds in the proliferation,attachment,and viability. Our founding indicated such nanofiber could be used as candidate scaffold in periodontal tissue engineering.