抗苗勒管激素及其Ⅱ型受体基因多态性与卵巢储备功能的关系

摘要

Objective To investigate gene polymorphism of the anti-Mullerian hormone (AMH) and its type JJ receptor (AMHRTJ)in polycystic ovary syndrome (PCOS) and premature ovarian fail ure ( POF). Methods Polymerase chain reaction (PCR)and DNA sequencing were used to detect the distribution of AMH and AMHR JJ genotype and allele frequency in 94 cases of PCOS,94 normal women and 32 cases of POF. The correlation between AMH and AMHR JJ genotypes with age, body mass index(BMI)and hormone levels was analyzed. Results ? There was significant difference in AMH and AMHR JJ genotypes ,and AMH allele frequency among three groups of subjects(P<0. 05) , but there was no significant difference in AMHR JJ allele frequency distribution(P> 0. 05) ;? In PCOS patients, AMH genotype was correlated with BMI,LH,T levels(P< 0. 05 ), while in the normal group and POF group, AMH genotypes were not associated with age, BMI, FSH, LH , T, E2 , PRL( P >0. 05);? In POF patients, AMHR H genotypes were associated with BMI,T and E2(P<0. 05),while in the normal group and PCOS group, AMHR JJ genotypes were not associated with age,BMI,FSH , LH,T,E2 ,PRL(P>0. 05). Conclusion AMH and AMHRJJ gene polymorphism may be associated with PCOS,POF.%目的 探讨抗苗勒管激素(AMH)及其Ⅱ型受体(AMHRⅡ)基因多态性与卵巢储备功能的相关性.方法 采用聚合酶链反应(PCR)及DNA测序方法,检测94例多囊卵巢综合征(PCOS)患者、94例正常排卵女性及32例卵巢功能早衰(POF)患者AMH和AMHRⅡ基因型及等位基因频率的分布情况,分析患者AMH、AMHRⅡ基因型不同与其年龄、体重指数(BMI)及激素水平的相关性.结果 ① 3组研究对象AMH、AMHRⅡ基因型及AMH等位基因频率分布存在差异(均P＜0.05),而AMHRⅡ等位基因频率分布无差异(均P＞0.05);②PCOS患者AMH基因型与BMI、LH、T水平相关(均P＜0.05),而正常对照组和POF患者AMH基因型与年龄、BMI、FSH、LH、T、E2、PRL水平无关(均P＞0.05);③POF患者AMHR基因型与BMI、T及E2水平相关(均P＜0.05),而正常组和PCOS患者AMHRⅡ基因型分布与其年龄、BMI、FSH、LH、T、E2、PRL水平均无关(均P＞0.05).结论 AMH及AMHRⅡ基因的多态性可能与卵巢储备功能异常有关.