Objective To study the cytotoxic effect of Artemisinin in different concentration on breast cancer MT40 cells and expression of CD71 and residual effect in vitro.Methods MT40 cells in logarithmic growth phase were divided into 20 ,40 , 60 and 80 μmol/L concentration Artemisinin groups and negative control group ,positive control group.Flow cytometry(FCM) was used to detect apoptosis and cell cycle change and the expression of CD71 of breast cancer MT40 cells after being treated by Artemisinin.The growth curve was drawn and the doubling time of surviving cells calculate.The experiment was repeated 3 times.Results Apoptosis rate of MT40 cells in 20 ,40 ,60 and 80 μmol/L concentration Artemisinin groups was (6.81 ± 1.28)%,(10.55 ± 3.49)%,(17.12 ± 3.73)% and(19.50 ± 3.70)%,respectively;the apoptosis rate of negative control and positive control was (1.15 ± 0.21)% and(2.45 ± 0.53)%,respectively;Artemisinin significantly increased apoptosis rate of MT40 cells(all P＜0.05).After MT40 cells were treated by Artemisinin in different concentration ,the cells at G0/G1increased and those at G2/M and S decreased(all P＜0.05).The expression of CD71 of MT40 cells was inhibited by Artemisinin in differ-ent concentration ,the expression rate of CD71 was(57.42 ± 3.45)%,(50.38 ± 3.22)%,(47.23 ± 2.35)% and (43.27 ± 2.86)%,respectively in 20 ,40 ,60 and 80 μmol/L Artemisinin group ,respectively;the expression rate of CD71 in negative con-trol was(62.90 ± 2.41)%,the expression rate of CD71 of positive control was(60.13 ± 2.89)%;Artemisinin inhibited the ex-pression of CD71 of MT40 cells in different concentration(all P＜0.05).The peak of the growth curve of surviving cells in each Artemisinin groups moved backwards ,the doubling time of the surviving cells in each groups was 0.59 ,0.59 ,0.61 ,0.65 ,0.71 , 0.78 days respectively in negative control ,positive control and 20 ,40 ,60 ,80 μmol/L Artemisinin group ,respectively. Conclusion Artemisinin can induce apoptosis ,block M T40 cells at G0/G1phase and depress the expression of CD71 and extend the doubling time in surviving cells in vitro.%目的 探讨不同浓度的青蒿素对乳腺癌M T40细胞的生长抑制作用和CD71表达的影响及后遗效应.方法将处于对数生长期的MT40细胞分为20 、40 、60 、80 μmol/L青蒿素实验组及不加任何药物的阴性对照组、10 μmol/L丝裂霉素的阳性对照组,采用流式细胞仪检测各组M T40细胞凋亡及细胞周期变化及CD71的表达,绘制各实验组后遗细胞生长曲线,计算细胞倍增时间,实验重复3次.结果 20 、40 、60 、80 μmol/L青蒿素实验组细胞凋亡率分别为(6. 81 ± 1. 28)%、(10.55 ± 3.49)%、(17.12 ± 3.73)%、(19.50 ± 3.70)%,阴性对照组为(1.15 ± 0.21)%,阳性对照组为(2. 45 ± 0.53)%,青蒿素明显提高MT40细胞凋亡率(均 P＜0.05).不同浓度青蒿素作用于MT40细胞后,G0/G1期细胞增加,G2/M及S 期细胞减少(均 P＜ 0.05). 20 、40 、60 、80 μmol/L 青蒿素实验组细胞 CD71表达率分别为(57.42 ± 3. 45)%、(50. 38 ± 3. 22)%、(47. 23 ± 2.35)%、(43. 27 ± 2. 86)%,阴性对照组为(62.90 ± 2. 41)%,阳性对照组为(60. 13 ± 2.89)%,青蒿素抑制了MT40细胞CD71的表达(均 P＜0.05). 20 、40 、60 、80 μmol/L青蒿素实验组后遗细胞生长曲线峰值后移(F＝ 11.86 ,P＜0.01).各组后遗细胞倍增时间,阴性对照组和阳性对照组均为0.59 d ,20 、40 、60 、80 μmol/L青蒿素组分别为0.61 、0.65 、0.71 、0.78 d .结论 青蒿素对乳腺癌M T40细胞具有诱导细胞凋亡,阻滞细胞于G0/G1期,下调细胞CD71表达的作用,并具有使MT40细胞倍增时间延长的后遗效应.
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