鱼肉中磺胺类抗生素的表面增强拉曼光谱探测与分析

摘要

In this paper,the silver colloid prepared by the microwave heating method was used as a Surface-enhanced Raman Scattering (SERS)active substrate for the trace-level detection of the sulfonamide antibiotics in fish.The extrac-tion effects of two extracts (ammonia water,ethyl acetate)had been compared.The results showed that the detection effect of ethyl acetate was better than ammonia water and the substances in fish had interference on the detection of anti-biotics.In this experiment,sulfamerazine and sulfamethazine were both detected with the lowest concentrations at 1 ppm by serving silver colloid as a SERS substrate and ethyl acetate as an extract,and the limits of detection were 0.16 ppm, 0.59 ppm,respectively.According to the results,the antibiotics in fish can be detected using SERS technology,and this work will provide an experimental foundation for the application of the detection of antibiotics in aquatic products.%本文以银溶胶为表面增强拉曼活性基底，实现了鱼肉中磺胺类抗生素的痕量检测。采用微波加热法制备银溶胶，比较了两种提取剂（氨水、乙酸乙酯）对鱼肉中抗生素的提取及探测效果。实验发现，鱼肉中的物质对抗生素检测有较大干扰，乙酸乙酯作为提取剂的效果要明显好于氨水。以银溶胶为基底，乙酸乙酯作为提取剂对两种限制使用的磺胺类抗生素（磺胺甲基嘧啶、磺胺二甲基嘧啶）检测的最低浓度皆为1 ppm，检测限分别为0．16 ppm、0．59 ppm。结果表明，利用此方法，可以实现鱼肉中一定浓度抗生素的检测，为实现水产品中抗生素的检测提供了实验基础。