目的 克隆树鼩(Tupaia belangeri)Retn基因,为在树鼩中开展Retn相关研究提供实验资料.方法 在树鼩脂肪组织中抽提总RNA,用RT-PCR进行基因扩增,通过基因克隆、重组质粒的酶切鉴定,对Retn克隆质粒的序列测定和分析.结果 抽提的总RNA完整性较好,RT-PCR产物电泳检测得到了目的条带,重组克隆质粒经Pst I单酶切证明了Retn基因已克隆至质粒载体,测序获得了363个核苷酸序列,1个编码114个氨基酸的开放阅读框,序列提交GenBank,登录号为JF267792；经Blast软件进行序列分析,其核苷酸序列和氨基酸序列与鼠类的同源性分别为95％和99％.结论 成功克隆了树鼩Retn基因及序列测定,为树鼩Retn基础数据的建立和开展相关研究提供了实验资料.%Objective The aim of this study was to clone the Retn gene of tree shrews to provide basic data for further research. Methods Total RNA was extracted from adipose tissue of tree shrews. Retn gene was amplified by RT-PCR and cloned. The sequence of Retn gene was identified by sequencing and enzyme digestion. Results The total RNA had good integrity. The expected 363 bp fragment was obtained by RT-PCR. The Retn gene was cloned in vector and identified by enzyme digestion (Pst I). 363 nucleotide acids encoded an open reading frame with 114 amino acids. The sequence has been submitted to GenBank (No. JF267792). The results showed a 95% similarity in nucleotide acids and 99% in amino acids with mouse Retn gene. Conclusion We have first reported the Retn gene of the tree shrew, and lay a foundation for further study on biological functions of Retn gene in tree shrews.
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