MicroPET观察姜黄素对APPswe/PS1dE9双转基因小鼠脑葡萄糖代谢的影响

摘要

目的 利用18氟-脱氧葡萄糖microPET( 18F-FDG microPET)影像学技术观察正常对照组小鼠,模型组和姜黄素治疗组APPswe/PS1dE9双转基因小鼠的脑葡萄糖代谢情况.方法 随机挑选6月龄正常对照组C57BI/6J小鼠3只,模型组和姜黄素治疗组APPswe/PS1dE9双转基因小鼠各3只,通过2％异氟烷吸入麻醉后,从尾静脉弹丸式注射放射性示踪剂18F-FDG每例约14.8～16.5 MBq,摄取45 min后进行10 min microPET图像采集.计算并比较各组小鼠每克脑组织(除小脑)18F-FDG的摄取率.结果 姜黄素治疗组小鼠每克脑组织18F-FDG的摄取率高于正常对照组和模型组.结论 姜黄素能明显提高APPswe/PS1 dE9双转基因小鼠脑18F-FDG的摄取及每克脑组织的摄取率,并可能通过影响脑葡萄糖代谢而发挥神经保护作用.%Objective To observe the status of cerebral glucose metabolism in normal control group mice, APP/ PS1 double transgenic mice of model group and curcumin treatment group by 18F-FDG microPET. Methods APP/PS1 dtg mice from the model group, curcumin treatment group,and normal C57BL/6J mice with the same background were randomly selected ( age: 6 months; n = 3 ) for microPET study. Each mouse was anesthetized using 2% isoflurane and was injected through a tail vein with 14. 8-16. 5 MBq "F-FDG as a bolus. After 45-minute of "F-FDG uptake period, PET scans were performed for 10-min. Calculate and compare "F-FDG uptake rate per gram tissue of the whole brain ( except cerebellum) in the mice of different groups. Results Cerebral "F-FDG uptake rate per gram tissue of curcumin treatment group mice is higher than both the normal control group and model group mice. Conclusions Curcumin can increase cerebral "F-FDG uptake and " F-FDG uptake rate per gram tissue. Curcumin may play a neuroprotective role through an influence to cerebral glucose metabolism.