通过MS-222对2种规格的异齿裂腹鱼进行麻醉行为特征研究,为高原鱼类的麻醉以及运输提供技术支持.试验表明:MS-222麻醉大规格(25.0±1.5) cm和小规格(14.8±2.3) cm异齿裂腹鱼时,在麻醉时期3期以内,呼吸频率增加并之间无显著性差异,在麻醉4期以后呼吸频率才开始显著下降.MS-222麻醉大规格和小规格异齿裂腹鱼的有效质量浓度分别为150-180 mg/L和150 mg/L.在此浓度范围内,大规格异齿裂腹鱼在MS-222麻醉液中,5min之内达到4级麻醉状态,5min之内苏醒恢复,且在麻醉液中浸浴20min后成活率为100％时的浓度;小规格异齿裂腹鱼在MS-222麻醉液中,5min之内达到4级麻醉状态,7min之内苏醒恢复,且在麻醉液中浸浴20min后成活率为100％时的浓度.大规格异齿裂腹鱼在180 mg/L的MS-222溶液中麻醉5min,在空气中进行暴露0-15min,复苏时间无显著性差异(P＞0.05);小规格异齿裂腹鱼在150 mg/L的MS-222溶液中麻醉5min,在空气中进行暴露0-15min,复苏时间存在显著性差异(P＜0.05).%This study examined anesthetic behaviors ofSchizothorax o'connori Llord in two size ranges as induced by MS-222,to acquire useful information on anesthesia of said highland fish species in out-of-water activities (surgery,labeling,measurement,etc.).Test results indicated that for both large (25.0±-1.5) cm and small-sized (14.8±2.3) cm specimens,respiration rates showed no significant change in anesthetic stages Ⅰto Ⅲ,and only begun to significantly decline after entering stage Ⅳ.The effective concentrations of MS-222 were 150-180 mg/L and 150 mg/L for two sizes,respectively.The 150-180 mg/L caused large specimens to enter anesthetic stage Ⅳ within 5min,and recovered within 5min;the 150 mg/L caused small specimens to enter stage Ⅳ within 5min,and recovered within 7min;both doses ensured a 100％ survival rate after immersion for 20min.For large specimens anesthetized at 180 mg/L for 5min and exposed to air for 0-15min,there was no significant difference in recovery times (P＞0.05);for small specimens anesthetized at 150 mg/L for 5min and exposed to air for 0-15min,there were significant differences in recovery (P＜0.05).
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