氧化豆油水溶物对离体草鱼肠道黏膜细胞的损伤作用

摘要

以氧化豆油水溶物为试验材料,在草鱼肠道黏膜细胞培养液中加入不同浓度氧化豆油水溶物,研究不同剂量氧化豆油水溶物在不同作用时间下对肠道黏膜细胞生长、细胞形态结构的影响。结果显示：添加111.06-888.48 g/L氧化豆油的水溶物作用6h显著降低细胞活性(P<0.05),细胞集落面积减小、细胞形态改变,其中在444.24 g/L 氧化豆油的水溶物作用下培养细胞空泡变性,且脂肪滴沉积,线粒体肿胀。在222.12-888.48 g/L氧化豆油的水溶物作用下,3-9h内培养液中LDH活力显著增高(P<0.05)；各时间点培养液中GSH-PX、SOD、T-AOC均有显著变化。结果表明,在培养液中添加111.06-888.48 g/L氧化豆油的水溶物作用12h 内,对草鱼肠道黏膜细胞产生了损伤,表现为抑制细胞生长,改变细胞形态、结构,可能引起细胞膜脂质过氧化,导致膜结构破坏,且作用程度与添加浓度、作用时间相关。研究认为氧化豆油水溶物对草鱼肠道黏膜细胞具有显著性的损伤作用。%The present study investigated the effects of water soluble matter from oxidized soybean oil on the growth, morphology and structure of intestinal epithelial cells (in vitro) from Ctenopharyngodon idella. The water soluble matter was applied at different concentrations for various periods of time. The activity and survival of cells were significant reduced after 6h incubation with culture medium supplemented with water soluble matter in the concentration range of 111.06-888.48 g/L. Furthermore there was an associated decrease in the size of cell colonies as well as a change in cell morphology. When the water soluble matter from oxidized soybean oil reached the concentration of 444.24 g/L, the formation of fat droplets and vesicles in cells could be observed under transmission electron microscope, as well as swelling mitochondria in cells. During 3-9h incubation, LDH activity significantly increased; there was also signi-ficant change in GSH-PX, SOD activity and T-AOC. In conclusion, during 12 hours the dissociated IECs from Cteno-pharyngodon idella could be damaged by the water soluble matter from oxidized soybean oil in the concentration range of 111.06-888.48 g/L. The damage occurs primarily in the manifestation of altered growth-inhibitory, morphology, and cell structure, probably involving the lipid peroxidation in cell membrane and the subsequent destruction in cell struc-ture. The extent of damage is correlated with the concentration and incubation time. Overall, there was significant damage of water soluble matter from oxidized soybean oil on IECs in vitro.