酵母培养物水溶物对离体草鱼肠道黏膜细胞生长及细胞膜完整性的影响

摘要

In this experiment, water soluble material of yeast culture as the experimental material was added in the culture solution of intestinal epithelial cells in vitro of grass carp ( Ctenopharyngodon idella) , in order to study the effects of water soluble material of yeast culture on growth and cell membrane integrity under the dif-ferent concentrations and different action time of water soluble material of yeast culture. One control group and 5 water soluble material of yeast culture groups ( YC-1 to 5 groups) were designed by using single-factor ex-perimental design, and each group had 96 replicates and 1 replicate was a culture hole. The control group was not added the water soluble material of yeast culture, and the concentration of water soluble material of yeast culture in YC-1 to 5 groups was 10, 25, 50, 100 and 200 mg/L, respectively. The results showed as follows:the cell morphology was no injury when adding 50 to 200 mg/L water soluble material of yeast culture into the culture solution, the cell activity was increased on 3 h after added 100 to 200 mg/L water soluble material of yeast culture, and the cell activity was increased on 6 h after added 50 mg/L water soluble material of yeast culture. Compared with control group, the activity of lactate dehydrogenase ( LDH) in culture solution in all water soluble material of yeast culture groups had no significant change on 3 h ( P>0.05) , while that in YC-4 and YC-5 groups was significantly reduced on 6 h ( P<0.05) , that in YC-3, YC-4 and YC-5 groups was sig-nificantly reduced on 9 h (P<0.05), and that in YC-2, YC-3, YC-4 and YC-5 groups was significantly re-duced on 12 h (P<0.05). The activities of glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase ( GOT) in culture solution in all water soluble material of yeast culture groups had no significant change on all time points (3, 6, 9 and 12 h) compared with control group (P>0.05), but the activity of GOP on 6 to 9 h in all water soluble material of yeast culture groups was lower than that in control group. In conclu-sion, adding 10 to 200 mg/L water soluble material of yeast culture into the culture solution can improve the growth and protect the cell membrane integrity of intestinal epithelial cells in vitro of grass carp, and the appro-priate concentration of playing protective effect is 50 to 200 mg/L.%本试验以酵母培养物水溶物为试验材料，添加于离体草鱼肠道黏膜细胞培养液中，研究酵母培养物水溶物在不同浓度、不同作用时间下对细胞生长及细胞膜完整性的影响。采用单因子试验设计，设1个对照组及5个酵母培养物水溶物组（ YC-1～5组），各组均设96个重复，每个重复为1个培养孔。对照组的培养液中不添加酵母培养物水溶物， YC-1～5组的培养液中酵母培养物水溶物的浓度分别为10、25、50、100、200 mg/L。结果表明：培养液中添加50～200 mg/L酵母培养物水溶物对细胞形态无损伤，100～200 mg/L酵母培养物水溶物在添加后3 h时显著增强细胞活性（ P＜0．05），50 mg/L酵母培养物水溶物在添加后6 h时显著增强细胞活性（ P＜0．05）。与对照组相比，3 h时各酵母培养物水溶物组培养液中乳酶脱氢酶（ LDH）活力均没有显著变化（P＞0．05），6 h时YC-4、YC-5组显著降低（P＜0．05），9 h时YC-3、YC-4、YC-5组显著降低（P＜0．05），12 h时YC-2、YC-3、YC-4、YC-5组显著降低（P＜0．05）。各时间点（3、6、9、12 h）各酵母培养物水溶物组培养液中谷丙转氨酶（ GPT）、谷草转氨酶（ GOT）活力与对照组相比均没有显著变化（ P＞0．05），但在6～9 h时GPT活力均低于对照组。由此得出，培养液中添加10～200 mg/L酵母培养物水溶物能促进离体草鱼肠道黏膜细胞的生长，保护细胞膜的完整性，其发挥保护作用的适宜浓度为50～200 mg/L。