南方灵芝中一种新型免疫调节蛋白的生物信息学分析

摘要

利用生物信息学方法从蛋白质基本理化特性、信号肽和亚细胞定位、分子进化与翻译后修饰位点以及蛋白质三维结构等方面,对南方灵芝(Ganoderma australe)中免疫调节蛋白c13717进行预测分析,并将c13717蛋白的分析结果与三种典型的真菌免疫调节蛋白(LZ-8、Fip-fve和Fip-vvo)以及牛樟芝(Antrodia camphorata)中免疫调节蛋白ACA进行比较分析.结果发现,c13717蛋白相对分子质量为14150,N端具有信号肽,亚细胞定位于细胞外,遗传进化与ACA相近,而与LZ-8、Fip-fve和Fip-vvo较远,翻译后修饰位点较少,蛋白三维结构含有cerato-platanin(CP)结构域,不具有真菌免疫调节蛋白典型的FNⅢ结构,证明c13717蛋白不属于真菌免疫调节蛋白(fungal immunomodulatory protein,FIP)家族,而属于CP蛋白家族,可能具有独特的生物活性.%Bioinformatic methods were used to determine various physicochemical characteristics,the signal peptide sequence,sub-cellular localization,evolutionary development,post-translational modification and the three-dimensional structure of c13717,a putative immunomodulatory protein from Ganoderma australe.Protein c13717 has a molecular weight of 14,150,is located extracellularly and contains a signal peptide,and phylogenetic analysis revealed it to be evolutionary closer to ACA,an immunomodulatory protein from Antrodia camphorata,and genetically distinct from LZ-8,Fip-fve and Fip-vvo,fungal immunomodulatory proteins (Fips) previously isolated from Ganoderma lucidum,Flammulina velutipes and Volvariella volvacea,respectively.Fewer post-translational modification sites were evident in c13717 compared to LZ-8,Fip-fve and Fip-vvo,and the 3D structure revealed the presence of a cerato-platanin (CP) domain rather than the typical FNⅢ structure found in LZ-8,Fip-fve and Fip-vvo.We propose that c13717 belongs to the CP family and may possess unique biological activity.