伸筋草正丁醇提取物对佐剂性关节炎大鼠血清IL-1β、IL-6、TNF-α的影响

摘要

目的：研究伸筋草正丁醇提取物对佐剂性关节炎(Adjuvant Arthritis，AA)大鼠血清中细胞因子IL-1β、IL-6和TNF-α的影响，探讨其治疗类风湿性关节炎(RA)的作用及免疫学机制。方法：采用弗氏完全佐剂(CFA)诱导大鼠AA模型，给予不同剂量的伸筋草正丁醇提取物治疗，应用放射性免疫法，检测血清中细胞因子系列(IL-1 β、IL-6、TNF-α)的水平。结果：模型组大鼠血清IL-1β、TNF-α、IL-6水平均高于正常组(P＜0.01)；各治疗组IL-1β、IL-6和TNF-α水平均有显著降低(P＜0.01或P＜0.05)。结论：伸筋草正丁醇提取物可能是通过免疫调节机制，对佐剂性关节炎发挥治疗作用。%Objective: To study the effects of butyl alcohol extracts from lycopodium clavatum ( EELC) on IL -1β, IL -6 and TNF - α in blood serum of adjuvant arthritis( AA) rats. Methods; The rats were immunized with the Freunds Complete Adjuvant (FCA). After treating the extracts from lycopodium clavatum were observed, and examine the IL-1β, IL - 6 and TNF-α by corresponding methods. Results: IL - 1β, IL - 6 and TNF - α levels of model group were increased compared with the normal group(P < 0.01); The IL - 1β, IL - 6 and TNF - α levels of each treatment group were decreased compared with the model group(P <0.01 or P <0.05). Conclusion; EELC can decrease and inhibit inflammatory disease to AA in rats through regulating immune mechanism.