抗痫灵对癫痫大鼠脑内nNOS活性影响的实验研究

摘要

Objective: To observe the effect of Kangxianling on the expression of nNOS in hippocampus of the PTZ - induced epilepsy model of rats, we probed into the efficacy and the mechanism of Kangxianling. Methods: PTZ was used to evoke chronic epilepsy model. Ethology changes and electroencephalography were observed after epileptic seizure,expressions of nNOS were investigated with Western blot method. Results: The groups of Kanxianling, VPA groups and PTZ-groups were significantly different from the control group (P <0. 05). The groups of Kanxianling was siginificantly different from the model group(P <0.01). Conclusion; Kangxianling has anti - epileptic seizure resistant function on PTZ -induced epilepsymodel of rats. Its function mechanism may be to react by reducing the content of nNOS in the hippocampus.%目的:观察中药抗痫灵对戊四氮点燃癫痫大鼠模型的影响,进一步探讨从调节一氧化氮合酶(nNOS)的nNOS(神经元型)亚型代谢的角度探讨抗痫灵的抗癫痫作用机制.方法:动物模型采用戊四氮点燃大鼠模型.免疫印迹( Western blot)方法观察实验各组大鼠大脑海马nNOS的活性变化.结果:抗痫灵组、丙戊酸钠组、模型组阳性细胞表达与正常对照组相比,有显著性差异(P＜0.05),模型组较正常对照组大鼠脑nNOS内活性显著升高(P＜0.01),中药组显著低于模型组(P＜0.01).结论:抗痫灵对戊四氮点燃大鼠癫痫发作有显著对抗作用,其作用机制可能与其降低模型大鼠脑内nNOS活性有关.