电针傍刺对褥疮大鼠创面修复的影响

摘要

目的:观察电针傍刺对褥疮大鼠创面愈合率、愈合时间及血流灌注量的影响.方法:用自制装置固定SD雌性大鼠制作褥疮模型,将150只大鼠随机分为正常组、模型组、电针组、模型抑制剂组和电针抑制剂组,每组按干预时间1d、3d、5d、7d、9d分为5个亚组,每个亚组6只.模型组给予碘伏常规治疗,电针组予以电针傍刺治疗,模型抑制剂组和电针抑制剂组于每日干预前30 min腹腔注射SB203580(5 mg/kg).观察创面愈合率和血流灌注量.另选24只褥疮大鼠,观察创面愈合时间.结果:3d、5d、7d、9d时,电针组创面愈合率大于模型组(P＜0.05);电针组创面愈合时间少于模型组(P＜0.05);造模后,褥疮大鼠创面血流灌注量高于正常组(P＜0.05),电针组在不同时间点治疗后创面平均血流灌注量高于治疗前(P＜0.05),电针组在1d、3d、5d、7d、9d时间点创面的血流灌注量呈下降趋势,早于模型组.结论:电针傍刺能够促进褥疮大鼠创面的愈合,缩短创面的愈合时间,增加创面的血流灌注量.%Objective:To investigate effect of Bangci(straight and side needling) with electro-acupuncture (EA) on the wound healing rate,the healing time,and blood flow peffusion of the local skin in rats with bedsore.Methods:The models of bedsore were set up by fixing SD female rats with self-made device.150 rats were randomly divided into the normal group,the model group,the EA group,the model inhibitor group,and the EA inhibitor group.Each group was randomly divided into five sub-groups according to different intervention time points,1 d,3 d,5 d,7 d and 9d sub-groups,with six rats in each sub-group.The model group was intervened with iodine;the EA group was given Bangci with EA;for the rats in the model inhibitor group and EA inhibitor group,SB203580(5 mg/kg) as p38 inhibitor was injected into them 30 min before daily intervention.The wound healing rate and blood flow perfusion of the local skin were observed.Another 24 bedsore rats were selected to investigate the healing time.Results:The EA group was better than the model group in terms of the wound healing rate on 3 d,5 d,7 d,9 d (P ＜ 0.05),and the healing time of the EA group was shorter than that of the model group (P ＜ 0.05).Blood flow perfusion of the local skin in bedsore rats were more than that of the normal rats (P ＜ 0.05);in the EA group,it became more after treatment at different intervention time than that before treatment(P ＜0.05),and it showed a gradual downward trend as intervention time goes on,which came earlier than that in the model group.Conclusion:The therapy of Bangci with electro-acupuncture can promote the wound-healing,shorten the healing time,and increase blood flow perfusion in bedsore rats.