莱菔硫烷对C57BL/6小鼠急性酒精性肝损伤的保护作用

摘要

目的:研究莱菔硫烷对酒精诱发的肝脏功能异常的保护作用.方法:雄性C57BL/6小鼠按体质量随机分为5组(n=12):阴性对照组、酒精模型组、SFN处理组(SFN的剂量分别为10 mg/kg、20 mg/kg、40 mg/kg),先给处理组动物经口灌胃不同剂量的SFN共10 d(1次/d),在末次灌胃SFN后,立即给予酒精模型组和SFN处理组动物酒精(5 g/kg),每12h1次,共3次,末次给予酒精4h后结束整个实验,常规HE染色观察肝脏病理变化,采用半自动生化分析仪检测血清、肝匀浆中相关指标的表达水平.结果:病理学和血清学指标表明,成功建立了小鼠急性酒精性肝损伤模型,莱菔硫烷能明显减轻酒精对肝脏的病理学损伤,高剂量莱菔硫烷保护组的作用明显,莱菔硫烷能保护酒精对AST、ALT、ALP 3种代谢酶功能的损伤,莱菔硫烷能提高肝匀浆抗氧化酶SOD和GSH-Px活性,降低脂质过氧化物MDA的水平.结论:莱菔硫烷能保护酒精对肝脏功能的损伤,其主要作用机制与SFN提高了酒精代谢的关键酶SOD和GSH-Px活性密切相关.%Objective:To investigate the effects of sulforaphane (SFN) on abnormal function of liver induced by alcohol.Methods:Male C57BL/6 mice were randomly divided into 5 groups (n =12),which were the negative control group,alcohol model group,and SFN pretreatment group (10 mg/kg,20 mg/kg,40 mg/kg).Mice were daily gavaged SFN with different dose for ten days.At the end of last intake of SFN,mice in groups of the alcohol model and SFN pretreatment were gavaged alcohol(5 g/kg) every 12 hours for 3 times.The whole experiment was ended after 4 hours of last alcohol intake.HE staining was performed to check the pathological changes of liver.Blood and liver homogenates were collected to measure the expression levels of the relevant indicators by semi-automatic biochemistry analyzer.Results:The acute hepatic injury model was successfully estabhshed in C57BL/6 mice,as shown by indicators of pathology and serum.SFN,in high dose especially could lessen the pathological damage induced by alcohol.Also SFN significantly inversed the activities of AST,ALT and ALP induced by alcohol.SFN obviously activated the anti-oxidant enzymes including SOD and GSH-Px.Additionally,SFN pretreatment could inhibit the alcohol-induced MDA level,products of lipid peroxidation in dose-dependent way.Conclusion:SFN can protect the functions of liver damaged by alcohol.The main mechanisms may be associated with enhancing the antioxidant-enzymes,which are in charge of the metabolisms of alcohol.