Objective:To investigate the effect of Jianpi Shexue prescription on the vital signs and organ index of ITP mice models,providing support for clinical application.Methods:The ITP models of BALB/c mice were established by injection of the anti-platelet serum (APS).Prednisone and Jianpi Shexue prescription were given,and then the changes of vital signs,the spleen and thymus indexes were detected.Results:①Mice body weight,body temperature had no significant change between the experimental group and control group (P ＞0.05).②On the 8th day,the swimming time between control group and ITP,prednisone,Jianpi Shexue prescription group had statistically significant differences (P ＜ 0.01);after the experiment,the swimming time of ITP group was still lower than that of the control group (P ＜0.05),and was prolonged in prednisone group and Jianpi Shexue prescription group,Jianpi Shexue prescription group had the best effect.③From the 4th day,compared with the control group,the food-intake of ITP group,prednisone group and Jianpi Shexue prescription group was decreased obviously (P ＜ 0.05);on the 10th and 12th days,the food intake of prednisone group was increased significantly compared with that of control group (P ＜ 0.05);on the 16th day,the food-intake of Jianpi Shexue prescription group was increased obviously compared with that of ITP group (P ＜ 0.05);④From the 6th day,the water consumption of ITP group was increased markedly compared with that of control group (P ＜ 0.05);From the 10th day,the water consumption of prednisone group was increased significantly compared with that of control group (P ＜ 0.05);the water consumption of Jianpi Shexue prescription group had no significant difference.⑤After administration of APS,the volume of spleen and thymus was enlarged obviously compared with that of control group (P ＜ 0.05);after administration of prednisone,the volume of spleen and thymus was reduced markedly compared with that of ITP group (P ＜0.01);the volume of spleen had no significant difference (P ＞0.05) and thymus was changed significantly in Jianpi Shexue prescription group (P ＜ 0.01).Conclusion:①The ITP model was consistent with the characteristics of the syndrome model.②Prednisone and Jianpi Shexue prescription can effectively improve the symptoms of easy fatigue,loss of appetite and other symptoms of spleen Qi deficiency.%目的:观察健脾摄血方对ITP小鼠生命体征和脏器指数的影响,为临床应用提供支撑.方法:以抗小鼠血小板血清(APS)免疫造模法,建立BALB/c小鼠模型,体内造成ITP小鼠模型,观察强的松、健脾摄血方对ITP小鼠生命体征和胸腺、脾脏指数影响.结果:①各实验组小鼠体质量、体温与空白对照组比较,无统计学意义(P＞0.05).②造模第8天,模型组、强的松组、健脾组小鼠游泳时间与空白对照组比较,有统计学意义(P＜0.01);实验结束后,模型组小鼠游泳时间仍低于空白组(P＜0.05),强的松组、健脾组小鼠游泳时间均有所延长,以健脾摄血效果最佳.③从注射APS第4天开始,模型组、强的松组、健脾摄血组小鼠进食量明显减少,与空白对照组比较,具有统计学意义(P＜0.05);干预给药第10天、第12天,强的松组模型小鼠进食量增加,与对照组比较,具有统计学意义(P＜0.05);第16天健脾摄血组小鼠进食量增加,与模型组比较,具有统计学意义(P＜0.05).④从注射APS第6天开始,模型组饮水量增加,与空白组比较,具有统计学意义(P＜0.05);给药第10天开始,强的松组小鼠饮水量增加,与空白组比较,具有统计学意义(P＜0.05),而健脾摄血组小鼠饮水量无明显增加.⑤注射APS后由于免疫效应,模型组脾脏、胸腺明显增大,与空白组比较,具有统计学意义(P＜0.05);经强的松干预后,脾脏、胸腺缩小,与模型组比较,具有统计学意义(P＜0.01).经健脾摄血方干预后,脾脏与模型组比较,无统计学意义(P＞0.05);胸腺具有统计学意义(P＜0.01).结论:①注射APS复制的ITP小鼠模型符合病证结合模型特征.②强的松、健脾摄血方对ITP模型小鼠不耐劳力、食欲不振等脾气虚症状有明显改善效果.
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