硫醇-磷脂混合双层膜的电化学性质及其与蜂毒素的相互作用研究

摘要

报道了硫醇-磷脂混合双层膜的循环伏安和电化学交流阻抗行为研究，并用电化学方法考察了蜂毒素与其相互作用.实验中通过冷冻表面沾有磷脂溶液的硫醇单层膜来制备混合双层膜.研究表明双层膜在电极表面形成致密的绝缘层，阻碍了电极表面的电子传递.在双层膜体系上引入的蜂毒素可在膜表面上形成孔洞，破坏膜的绝缘性，降低膜电阻，增加膜电容，使带负电的探针Fe(CN)63-的氧化还原反应速度加快.%Hybrid bilayer membranes were fabricated on monolayer of alkanethiols self-assembled on gold electrode by freezing the monolayer coated with lipid solution for a few minutes. The resulted bilayer membranes were characterized with cyclic voltammetry and ac impedance. The interaction of a pore-forming peptide,melittin with such bilayer membranes was investigated with electrochemical techniques. The presence of hybrid bilayer membranes on electrode shows remarkable insulating properties by greatly attenuating the electron transfer between soluble redox couple. Adsorption of melittin into HBM destroys the insulating capability of HBM, causing membrane resistance to decrease and membrane capacitance to increase, thus accelerating the redox reaction for the negatively charged redox couple Fe(CN)63- .