利用Caco-2细胞单层模型和超高效液相色谱-三重四极杆电喷雾质谱联用方法,考察附子中乌头类生物碱提取物的跨膜转运.在相同浓度的附子乌头类生物碱溶液中,加入干姜、甘草、半夏及浙贝母的水提物（折合药材质量比例为1∶1）,系统考察了加入前后的表观渗透系数（Papp）.结果表明,加入配伍药材后附子中11种二萜类生物碱的Papp发生了明显的变化.干姜、甘草和半夏水提物的加入能够明显地降低二萜类生物碱在小肠中的生物利用度,尤其抑制其中毒性较大的双酯型生物碱的生物利用度;而浙贝母的水提物却在促进吸收和抑制外排两个方面%A UPLC/MS method has been established to analyze the diterpenoid alkaloids transportation in the Aconitum carmichaeli with the Caco-2 cell monolayer.Different combination herbs’ water extracts in-cluding Radix Glycyrrhizae,Rhizoma Zingiberis,Rhizoma Pinelliae or Bulbus Frltillariae Thunbergii have been mixed with the same concentration of the Aconitum carmichaeli alkaloids extract solution.The mixing ratio was converted into their medicinal herbs mass of 1 to 1.The results demonstrated that the bioavailabil-ity of the diterpenoid alkaloids especially the diester-diterpenoid alkaloids has been decreased by the adding of the Radix Glycyrrhizae,Rhizoma Zingiberis or Rhizoma Pinelliae extracts,which are the most toxicity compounds in the Aconitum carmichaeli.However,the bioavailability of the diterpenoid alkaloids in the absorption and efflux has been increased by the adding of the Bulbus Frltillariae Thunbergii extract,respec-tively.
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