以秋水仙素处理‘金星无核’葡萄获得的短节间突变体植株‘3-2D-05’和‘金星无核’植株新抽枝梢嫩叶、梢尖和茎段为材料,利用高效液相色谱(HPLC)法测定了新抽枝梢不同部位内源GA3、IAA及ABA的含量及比值差异；采用细胞流式仪分析两种材料叶细胞染色体倍性,并通过RAPD和SSR两种标记对两种植株进行分子鉴定,以明确两者的遗传差异.结果显示:(1)与‘金星无核’相比,突变体‘3-2D-05’植株嫩叶、梢尖和茎段中的内源GA3、IAA和ABA含量均降低；‘3 2D-05’植株嫩叶、梢尖和茎段中的内源GA3、IAA和ABA含量总和分别为‘金星无核’的29.58％、20.04％和20.03％,GA3/IAA值分别为‘金星无核’的1.30倍、0.97倍和0.89倍.(2)两种材料叶片细胞的染色体倍性一致,DNA序列差异不大,其遗传背景表现出高度一致性.研究表明,秋水仙素处理‘金星无核’获得的染色体倍性未发生变化的葡萄短节间变异与调控内源激素的基因有关,秋水仙素诱发了基因突变,产生了节间变短的葡萄新种质.%A short internode mutant plant (3-2D-05) was discovered from the progeny of 'Venus seedless' grape after treatment with colchicine. Taking young leaves,shoot tips and stems from new developed branches of 'Venus seedless' grape and ' 3-2D-05 ' as materials, we determined the endogenous gibberellin (GA3) , indoleacetic acid (IAA) and abscisic acid (ABA) by high performance liquid chromatography (HPLC) and testecd chromosome ploidy of their leaves by flow cytometry. RAPD and SSR were also used to determine 'Venus seedless' and ' 3-2D-05 ' on molecular level. The results showed that the contents of GA3 ,IAA and ABA in the young leaves, shoot tips and stems of '3-2D-05' were all lower than those of 'Venus seedless' grape. The sum of GA3 ,IAA and ABA in the three positions of the mutant plant accounted for 29. 58% ,20. 04% and 20.03% of the 'Venus seedless' grape, respectively; And the ratio of GA3/ IAA accounted for 1. 30,0. 97 and 0. 89 times, respectively. Meanwhile, the chromosome ploidy did not chang between the two materials by ploidy analysis. The PCR results showed that they had little differences in DNA sequences and high consistency in the genetic background. The short-internode variation generating without chromosome ploidy changing by colchicine was related to the genes that regulate the endog-enous hormone contents. We gained the new short-internode mutant plant from genes mutation induced bycolchicine.
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