以万寿菊‘钻石’雄性不育株的幼嫩叶片和子房为外植体进行离体快繁,结果表明:培养基MS+0.8 mg·L-1 IAA+0.6 mg·L-1 6-BA+30 g·L-1蔗糖既有利于叶片愈伤组织的诱导也有利于不定芽的分化,愈伤组织诱导率达97.9％,不定芽诱导率达45.8％；培养基MS+0.5 mg·L12,4-D+0.5 mg·L-16-BA+ 30 g·L-1蔗糖为诱导子房愈伤组织的最佳培养基,出愈率为77.8％；培养基MS+0.05 mg·L -1 NAA+0.5 mg·L-1 6-BA+ 30g·L-1蔗糖为诱导子房愈伤组织不定芽分化的最佳培养基；将不定芽接至MS培养基上,7d后即可生出不定根,生根率可达98％,移栽成活率达90％以上.%Immature leaves and ovaries from mature plant were used as explants for the regeneration of Tagetes erecta L. 'Diamond' male sterile plants in vitro culture. The best medium to induce callus and adventitious buds from leaves was the medium:MS+0. 8 mg · L-1 IAA+0. 6 mg · L-1 6-BA+30 g · L-1 sucrose. The callus and buds induction rate was 97. 9% and 45. 8%, respectively. The best medium for the callus induction from immature ovaries was the medium;MS+0. 5 mg · L-1 2,4-D+0. 5 mg · L-1 6-BA + 30 g · L-1 sucrose. The best medium for adventitious bud differentiation of ovary callus was the medium: MS+0. 05 mg · L-1 NAA+0. 5 mg · L-1 6-BA + 30 g · L-1 sucrose. When adventitious buds were transferred to the MS medium,98% of the buds could grow adventitious roots 7 days later. More than 90% of the rooted shoots survived after transferred into the soil.
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