从棉花纤维cDNA中克隆获得乙烯合成基因GhACO3,构建了植物过量表达载体p35S::GhACO3.通过花序侵染法和叶盘法分别转化拟南芥和烟草,利用卡那霉素筛选及分子检测获得转基因阳性拟南芥和烟草植株.结果表明,GhACO3基因已整合到拟南芥和烟草基因组中；经过纯合筛选后获得转基因T2代拟南芥植株；与野生型拟南芥相比,GhACO3基因对拟南芥不定根发生具有显著促进作用；与野生型烟草植株相比,转GhACO3基因烟草不定根发生得到了显著的促进.研究表明,GhACO3基因的过量表达能够促进拟南芥和烟草不定根的形成发育,为进一步探讨GhACO3的生物学功能和进行转基因育种奠定了基础.%Ethylene signaling plays important roles in multiple processes of roots growth and development, 1-aminocyclopropane-l-carboxylicacid oxidase (ACO) was the key gene in ethylene synthesis. We cloned a cotton GhACO3 gene from cotton fiber cDNA and constructed a plant overexpression vector p35S :: GhACO3. Genetic transformation of Arabidopsis and tobacco were performed by floral dip and leaf disk transformation method,respectively. The successive selection were completed with kanamycin and further molecular identification of transgenic Arabidopsis and tobacco plants. The results indicated that,GhACO3 gene was integrated into Arabidopsis and tobacco genomes,and the positive transgenic plants were obtained successfully. The T2 generation homozygous transgenic Arabidopsis plants were obtained by self-crossing, and with the comparison of Col wide type plants, adventitious roots of transgenic GhACOS Arabidopsis plants were promoted significantly. Also,compared with wild-type tobacco plants,the adventitious root development of transgenic GhACOS tobacco plants were also promoted significantly. The results showed that overexpression of GhACO3 promoted adventitious root development of Arabidopsis and tobacco. This work establishes the basis of further research for biological functions of GhACO3 and transgenic breeding by genetic engineering technology.
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