为了明确不同启动子驱动glgC基因表达的差别,对GBSS Ⅰ和35S两个启动子分别驱动的glgC在马铃薯转化株系的表达进行了检测,并测定了转化株系淀粉积累的表型差异.结果显示:两个启动子驱动的glgC在各自的转化株系中均实现了表达,且单拷贝glgC转化株系的AGPase活力、块茎淀粉含量和其粘度的表达均显著高于对照,其中GBSSⅠ:glgC转化株系的块茎淀粉含量和粘度均是对照的2倍以上,但其直链淀粉含量显著低于对照.研究表明,不同启动子驱动glgC植物体内表达,可实现其淀粉生物合成的调控.%To find out difference of promoters to drive glgC expression in transgenic plants,glgC expression driven by GBSS I and 35S,respectively,in potato transgenic lines was screened in this study. Phenotype on tuber starch accumulation was assayed in transgenic potatoes. The result showed that glgC driven either by GBSS I or 35S did express in the transgenic lines. AGPase activity, tuber starch contents and starch viscosity in the transgenic lines expressing single copy of glgC were significantly higher than those in control. Tuber starch contents and starch viscosity in GBSS I : glgC line were 2 time higher than those in control. Amylose contents in GBSS I : glgC line was significantly lower than that in control. The result indicated that starch biosynthesis can be regulated by glgC expression driven by different promoters.
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