不结球白菜BcLRK01基因对芜菁花叶病毒及胁迫诱导的响应

摘要

通过cDNA-AFLP技术,从芜菁花叶病毒(TuMV)侵染的不结球白菜幼叶中分离到一条差异表达的基因片段,克隆获得其cDNA全长为2124 bp,编码707个氨基酸的富亮氮酸重复类受体激酶,命名为BcLRK01.利用实时定量PCR研究了该基因在TuMV侵染及高盐、冷胁迫、水杨酸(SA)、茉莉酸(JA)、乙烯(ET)等处理下的表达情况,结果显示,TuMV侵染、高盐、冷胁迫、SA、JA和ET等均能诱导BcLRK0l不同程度的表达,说明该基因可能是不结球白菜病毒病的病程相关基因,同时也参与高盐和冷胁迫以及SA、JA、ET等的信号途径.%A differentially expressed gene fragment was isolated from TuMV-infected seedling leaves of non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) through cDNA-AFLP technique. The full-length cDNA of this gene,named BcLRKOl ,is 2 124 bp in size and encodes a leucine-rich repeat receptor-like kinase with 707 amino acids. Real time PCR was performed to detect its expression patterns under TuMV infection and the inductions of high salinity,cold,salicylic acid (SA) ,jasmonate acid (JA) and eth-ylene (ET). The results showed that all the treatments induced the expression of this gene significantly in different extents,which indicate that it may be a pathogenesis-related gene of virus disease in non-heading Chinese cabbage and involves in the signaling pathways of high salinity,cold,SA,JA and ET.