Seven compounds were isolated from the fruiting bodies of Hydnellum sp. No. 2 by silica gel and Scphadex LH-20 column chromatography. With combine analysis of spectral data and literature,their structures were identified as ergosta 7, 22-dien-3β-ol (1), ergosterol peroxide (2), (22E, 24R)-3β-hydroxy-24-meihylcholesta-5,22-dien-7-one (3) ,nipagin (4), (24R)-ergosta-7,22-diene-3β,5α,6β-triol (5) ,tuberoside (6) ,and cerebroside B (7). Compounds 4,5,6 and 7 were obtained from this genus for the first time.%利用硅胶柱层析和Sephadex LH-20凝胶柱层析分离手段,从高等真菌齿菌(Hydnellum sp.No.2)子实体的甲醇提取物中分离鉴定了7个化合物,经现代光谱学技术鉴定为麦角甾-7,22-二烯-3β-(1)、5α,8α-过氧麦角甾-6,22-二烯-3β-醇(2)、(22E,24R)-3β-羟基-24-甲基胆甾-5,22-二烯-7-酮(3)、对羟基苯甲酸甲脂(4)、(24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(5)、Tuberoside(6)和脑苷脂B(7).化合物4、5、6、7为该属首次报道.
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