结缕草是优良的暖季型草坪草之一，主要用于亚热带和热带地区的草坪种植。抗冷性是结缕草栽培范围的限制因子。本研究以日本最北部原产的结缕草品系为材料，根据其他植物的已知的抗寒基因 CBF 序列，通过同源克隆的方法获得结缕草中相对应的同源基因ZjCBF；根据和其他已报告的CBF序列的比对结果，确定ZjCBF基因属于CBF转录因子家族基因中CBF1型基因。利用半定量PCR和实时定量PCR分析该基因在寒冷条件下的表达情况，发现ZjCBF基因受冷胁迫的诱导，在4℃处理6 h时表达量最高。在此基础上，本研究构建了该基因的过表达载体，并将其转化到拟南芥中，通过低温冷处理实验发现，不论是否经过冷驯化，转 ZjCBF 基因植株由于 ZjCBF 的过量表达均比野生型植株抗寒性强。%Zoysiagrass is recognized as an excellent warm-season turfgrass and mainly used in subtropical and tropical regions. Cold stress is a major constraint factor for the cultivation of zoysiagrass. In this study, according to the sequences of cold tolerance gene CBF had been reported in other plant species, we cloned the corresponding homologous of the ZjCBF gene by homology cloning method in Zoysia japonica using a material originated from the most northern area of Japan. Based on the alignment re-sults compared with other reported CBF genes, we found the ZjCBF gene belongs to the CBF1 familiy. By semi-quantitative PCR and real-time quantitative PCR, we analyzed the expression level of the ZjCBF gene in the cold condition and found that ZjCBF was induced by cold stress, and the ZjCBF expression reached peak at six hours post 4°C treatment. In addition, we also con-structed ZjCBF over expression vector and generated transgenic Arabidopsis plants, with better cold tolerance than the wild-type, whether through cold acclimation or not.
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