DTA-6对大豆花荚脱落纤维素酶和GmAC基因表达的调控

摘要

It is effective to regulate abscission of soybean flowers and pods by spraying plant growth regulators (PGRs). This study was carried out to determine the effect of DTA-6 on abscission cellulase (AC) activity, abscission cellulase gene (GmAC) expressive, abscission of flowers and pods, and yield in soybean. DTA-6 was foliage sprayed at R1 stage on three varieties of Suinong 28, Kenfeng 16, and Hefeng 50 in 2012 and 2013. The results demonstrated that DTA-6 treatment inhibited GmAC gene expression in abscission zone of flower and pod, with the maximum reduction of 51%among three varieties compared with the control. The abscission cellulase activity was periodically decreased by DTA-6, with different decrements among soybean culti-vars. DTA-6 significantly decreased (P<0.05) abscission rate of soybean flowers and pods, and significantly increased yield (P<0.05). The relative expression of abscission cellulase gene (GmAC) in abscission zone of flowers-pods was decreased and the AC activity was regulated by DTA-6, resulting in reduced abscission rate of soybean flowers and pods and promoted yield.%叶面喷施生长调节剂可以调控大豆花荚脱落。2012—2013年在大庆林甸县黑龙江八一农垦大学试验基地采用大田小区试验， R1期对大豆绥农28、垦丰16和合丰50分别叶面喷施DTA-6调节剂，对调控花荚离区脱落纤维素酶(GmAC)基因相对表达量、荚脱落纤维素酶(AC)活性、花荚脱落率和产量进行了研究。结果表明： DTA-6抑制大豆花荚离区GmAC基因相对表达量，最大可达51%(±CK%)；大豆荚的AC活性能够在喷药后阶段性地被降低，在不同品种上增加和降低幅度存在差异；能够显著降低花荚脱落率(P<0.05)，显著增加产量(P<0.05)。植物生长调节剂DTA-6是通过降低花荚离区GmAC基因相对表达量，调节AC活性，从而减少植株的花荚脱落率，以实现对产量的调控。