萘乙酸对董棕变温冷胁迫的生理效应

摘要

为了研究不同浓度萘乙酸（NAA）溶液对董棕（Caryota obtusa）幼苗耐寒性的影响，分别用0，25，50和100 mg· L-14种浓度的NAA溶液喷施董棕叶片，并分别经过25℃-5℃-25℃各24 h变温冷胁迫处理后，研究董棕叶片在变温冷胁迫过程中超氧化物歧化酶（ SOD）、过氧化物酶（ POD）、丙二醛（ MDA）、脯氨酸、可溶性蛋白和叶绿素含量等生理指标的变化规律，并对25℃恢复24 h后的董棕幼苗上述耐寒性指标进行模糊隶属函数综合评价，结果表明：4种浓度下隶属函数值分别为0.39，0.30，0.39和0.51，其中喷施100 mg· L-1的NAA溶液对提高董棕的耐寒性效果最好。%The effects of naphthalene acetic acid ( NAA ) on the cold tolerance of Caryota obtusa seedlings were conducted by several physiological indexes , The leaves of C.obtusa were sprayed with 0, 25, 50 and 100 mg· L-1 NAA solutions , the activities of superoxide dismutase ( SOD ) , peroxidase ( POD ) , and the contents of malondialdehyde (MDA), proline, soluble protein and chlorophyll of C.obtusa were determined under 25℃, 5℃and 25℃.It was indicated that all the above physiological indexes varied with different concentrations of NAA . Subordinate function values for different concentrations of NAA at 25℃ were 0.39 , 0.30 , 0.39 and 0.51 , respectively .It was concluded that leaves sprayed with different concentrations of NAA could affect the cold tolerance of C.obtusa seedlings and the optimal concentration was 100 mg· L-1 .