比较了3种提取液制备红颊、川九1号、吉林草莓细胞核悬液流式细胞术检测效果，结果显示，提取液Ⅲ主峰粒子团清晰而集中，背景碎片少，试剂配方简单配制容易，且3种草莓材料倍性差异区分明显，优于其他2种提取液。对红颊草莓不同部位或不同来源的材料进行流式细胞术检测效果比较，结果表明，组培苗的检测效果明显优于大田试材，组培苗叶片因取材容易、主峰清晰、杂峰少、细胞碎片少而成为最佳试材。%Effect of three kinds of extraction buffers on preparation of strawberry nuclei suspension flow cytometry of Hongjia, Chuanjiu No.1 and Jilin was investigated.The results showed that extraction bufferⅢ had clear and focused peak particle , little background debris , simple and easy-to-prepare formula and apparent differences distinguished among three kinds of strawberries materials , which was better than the other two kinds of extraction buffers.Comparison of testing effect on strawberry Hongjia materials with different parts or different sources was performed.Detecting effects of plantlets were superior to field testing materials .Leaves of plantlets were the best test materials due to easy material availability , clear peak , less miscellaneous peak and less cellular debris .
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