In order to explore the synergistic toxicity of phthalate acid esters (PAEs), the bone marrow micronucleus formation, sperm deformity, liver and testis damage were carefully observed and determined on male mice , which were exposed to mixed PAEs chemicals with different levels of dose (40, 400, 4 000 mg· kg-1 ) and single dibutyl phthalate (DBP) with the same doses (40, 400, 4 000 mg· kg-1).It was shown that the bone marrow micronucleus formation rate and sperm deformity rate were obviously higher in the mixed group with high -dose (4 000 mg· kg-1 ) PAEs than those in the single DBP treatment .Besides , the damage of liver and testis was also higher in the mixed group with moderate-dose (400 mg· kg-1 ) PAEs than that in the single DBP treatment after 30 d.As mentioned a-bove, the mixed PAEs exhibited more severe toxic effects than single DBP treatment , which suggested that there might be certain synergistic effect among PAEs chemicals .%为了探讨邻苯二甲酸酯( phthalic acid esters , PAEs)类化合物的联合毒性作用,将6种PAEs类化合物配制成等质量比的混合物,以低、中、高剂量(40,400,4000 mg· kg-1)对雄性小鼠灌胃染毒,观察雄性小鼠骨髓微核形成、精子畸形、血清及肝脏、睾丸等指征的变化,并与相同剂量处理的单一邻苯二甲酸二丁酯( DBP )组进行比较,以评价混合PAEs组的联合毒性作用。结果显示:高剂量处理时,混合PAEs组的雄性小鼠骨髓微核率及精子畸形率显著高于单一DBP组;中剂量处理时,混合PAEs组染毒雄性小鼠30 d后的肝损伤及睾丸组织损伤程度也较单一DBP组明显上升,表明混合PAEs组对雄性小鼠的联合致毒作用较单一DBP组显著,不同PAEs类化合物间可能存在一定的协同效应。
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