利用类番茄茄(Solamum lycopersicoides Dun.)无菌苗幼嫩茎段酶解获得大量原生质体(个)(2×106/g)，将原生质体密度稀释至1×105/mL于HMA培养基中培养，则10d左右出现小细胞团，38～40d形成肉眼可见的小愈伤组织(1～1.5mm)，转移至MC增殖培养基2周后，再转移到15#分化培养基诱导出芽，切取芽体转入50P培养基诱发根原基，再转入50#发根培养基形成发达根系，成为再生植株，整个再生周期80～90d，再生植株移植至土壤中均能正常生长、开花。%A procedure for protoplast isolation, culture and plant regeneration has been developed for solanum lycopersicoides. Stem－protoplasts were diluted to 1×105/mL and cultured in HMA medium, cell colonies (20～30 cells) appeared after 10 days, micro calli (1～1.5mm) formed after 38～40 days from initially culturing. The micro calli were transferred to MC greenig medium for 2 weeks. The green calli were transferred to 15# medium for shoot inducting. The shoots which excised from the callus were transferred to test tubes with 50p medium first for root promoting about 3～4 days and then transferred to 50# rooting medium. Plants regenerated after 80～90 days after initially culturing. All the plants which potted in soil could vigorously grow and blossom.
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