冬季大叶黄杨光合电子传递动力学室内恢复机制研究

摘要

By repairing the overwingtering Euonmymus japonicus leaves in room, the electron transport kinetics between photosystem II (PS H ) and photosystem I (PS I ) were studied with Daul-PAM100 chlorophyll fluorescence and P700 redox synchro - analyzer. The results indicated that the PS II efficiency of shade leaves was higher than that of sun leaves, while the PS I efficiency of sun leaves was higher. When the two kinds of leaves were transferred to room, both OJIP and P700 redox kinetics refreshed gradually, the repairing process of both PS II and PS I included two steps, while they were not synchronic. The quick step of PS II was from 0 h to 43 h, whereas that of PS I was from 0 h to 6 h. The Pm times were brought forward from 20 ms to 2 ms as the leaves were transferred in room. In a sum, refreshing process of electron transport of the sun leaves was different from that of the shade leaves.%通过Daul-PAM 100叶绿素荧光-P700氧化还原动力学同步分析系统,研究冬季大叶黄杨(Euonmymus japonicus)室内恢复过程,光系统Ⅱ(PSⅡ)和光系统Ⅰ(PSⅠ)电子传递动力学的转变机制.结果表明:冬季逆境条件下,遭受低温和强光双重胁迫的阳生叶PSⅡ光量子效率低于仅遭受低温胁迫的阴生叶,但阳生叶PSI量子效率Y(Ⅰ)高于阴生叶.室内恢复过程,阳生叶与阴生叶的OJIP荧光动力学和P700氧化还原动力学活性逐渐恢复,PSⅡ与PSⅠ恢复过程均分快速阶段和慢速阶段,但两个光系统并不同步,PSⅡ快速恢复阶段为0～43 h,PSⅠ为0～6h,阳生叶室内恢复43 h,阴生叶室内恢复6h后,Pm峰值出现时间由20 ms提前至2 ms.由此可见,冬季室内恢复过程中,大叶黄杨阳生叶与阴生叶的两个光系统间的电子传递发生了不同的转变.