为选育出抗根肿病DH材料,本研究通过菌土接种法筛选出对根肿病具有较好抗性的白菜王后代C08384、C08385,病情指数分别为4.18和7.67.采用小孢子培养技术培养出DH系并对该体系进行优化.当离心转速1 200 r/min、热激温度32.5℃,处理1d时,最有利于胚状体的诱导；培养基中琼脂质量浓度为1.2％时,有利于诱导胚再生成幼苗；当培养基中激素质量浓度是6 - BA 1.0 mg/L+ NAA 0.1 mg/L时,有利于不定芽的诱导.%In order to breed DH materials with resistance to clubroot, with bacterium soil inoculation method of root C08384, C08385 of the cabbage queen generation with strong resistance against clubroot were developed by selection. Their disease indices were 4.18 and 7.67 respectively. Using the microspore culture technology the DH system was developed and optimized. The conducive conditions for cultivation of cabbage microspore embryo,were centrifugal speed, 1 200 r/min, heat -shock temperature, 32.5 ℃ and treating time one day. 1.2% of AGAR concentration of medium was conducive to induction of embryos into seedlings. 6 -BA1.0 mg/L + NAA 0.1 mg/L of medium hormone concentration was conducive to seedling induction.
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