Annual hardwood and softwood cuttings in 3-year-old cutting orchard of tetraploid Robinia pseudoacacia were taken as materials in this experiment. The changes of soluble protein content, soluble sugar content, starch content, total nitrogen content and C/N ratio were studied to provide reference for chemical control of the cuttings of tetraploid Robinia pseudoacacia. The result showed that; (1) the soluble protein content of control cuttings kept declining during rooting process. The soluble protein content of IBA-treated cuttings decreased firstly, and then increased. The soluble protein content of hardwood cuttings pretreated with IB A was significant higher than that of the control. (2) However, the soluble sugar contents of both hardwood and softwood cuttings showed a trend of increasing firstly, and then decreasing. The soluble sugar content of IBA-treated cuttings was higher than that of the control, but showed no significant difference. The highest soluble sugar content of IBA-treated cuttings appeared five days earier than that of the control. (3) The IBA-treated cuttings had a larger speed to degrade starch than the control. At the same time, the cuttings pretreated with IB A showed significant difference. (4) There was little relationship between total nitrogen and the rooting of the cuttings. (5 ) A positive relationship was detected between C/N ratio and the rooting percentage of tetraploid Robinia pseudoacacia cuttings.%取四倍体刺槐3年生采穗圃中1年生硬枝和半木质化嫩枝枝条为试材,研究硬枝和嫩枝插条不定根发生过程中可溶性蛋白、可溶性糖、淀粉和植物总氮含量及C/N比值的变化规律,为四倍体刺槐扦插生根的化学调控提供参考依据.结果表明:(1)不定根形成过程中,对照插穗可溶性蛋白含量持续下降；经IBA处理的插穗可溶性蛋白含量先降低后升高,且经IBA处理的硬枝插穗可溶性蛋白含量显著高于对照.(2)插穗可溶性糖含量先升高后降低；经IBA处理的插穗可溶性糖含量明显高于对照,且可溶性糖含量达到高峰的时间较对照提前5d.(3)经IBA处理的插穗淀粉含量下降的速度大于对照,且经IBA处理的硬枝插穗和对照之间差异水平显著.(4)植物总氮对四倍体刺槐扦插生根的影响作用较小.(5)四倍体刺槐扦插,插穗C/N比值越高,生根能力越强.
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