为研究多噬伯克霍尔德氏菌Burkholderia．multivorans WS－FJ9的解有机磷机制，对该菌磷酸酶产生的细胞定位进行了探讨并对其降解有机磷的营养条件进行了优化。采用冰浴超声破碎及液体培养的方法对WS－FJ9胞外、胞内及膜周质提取液的磷酸酶活性及不同营养条件下磷酸酶活性和解磷量分别进行了测定。结果表明：多噬伯克霍尔德氏菌Burkholderia．multivoransWS－FJ9能分泌酸性和碱性磷酸酶且以分泌酸性磷酸酶为主，均定域为胞外酶；该菌株解有机磷的最适碳、氮和磷源分别为葡萄糖、硫酸铵和卵磷脂，降解培养基中卵磷脂的最适浓度为0．5 g／L。研究结果为生物菌肥的开发与利用提供了理论参考依据，对可持续农业的发展具有重要意义。%Phosphatase cellular localization was investigated and optimization of nutritional conditions was conducted for the purpose of studying the organophosphorus-degrading mechanism of Burkholderia multivorans WS-FJ9.The phosphatase activity of the extracellular,the intracellular and the membrane periplasm extracted solution,and both the phosphatase activity and organophosphorus-degrading quantity under different nutritional conditions were determined respectively using the methods of submerged culture and ice bath and ultrasonic breaking.The results showed that, B.multivorans WS-FJ9 was able to secrete acid phosphatase ( ACP ) and alkaline phosphatase ( AKP ) ,which were all localized as extracellular enzymes,and ACP was the principal component.The optimal carbon,nitrogen and phosphorus sources for degrading organophosphorus were glucose, ammonium sulfate and lecithin,respectively.The optimal concentration of lecithin was 0.5 g/L in the organophos⁃phorus-degrading culture medium.This study provides a theoretical reference basis for the development and utili⁃zation of bio-bacterial fertilizers,and is of great significance for the development of sustainable agriculture.
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