厚朴苗的组织培养研究

摘要

采用正交试验及单因素随机区组设计，运用极差分析、方差分析、LSD多重比较分析结果，对厚朴苗组织培养的芽诱导培养、增殖培养、生根培养、壮苗培养、移栽炼苗各阶段最佳培养基组合进行探讨。试验表明：芽诱导的B5＋6-BA 5．0 mg/L＋NAA 1．0 mg/L＋2，4-D 1．0 mg/L组合最佳；增殖培养B5＋6-BA 5．0 mg/L＋NAA 0．1 mg/L＋蔗糖15 g/L组合最好；壮苗效果B5＋NAA 0．5 mg/L＋GA30．3 mg/L组合的芽苗生长健壮；生根培养B5＋IBA 1．0 mg/L＋NAA 1．0 mg/L的生根率最高，达62．7％，ABT1、ABT3也可诱导生根，B5＋ABT31．5 mg/L＋蔗糖20 g/L组合生根率高达61．2％；自然光闭瓶锻炼6 d再开瓶炼苗6 d，移栽成活率高达92．2％，移栽基质为沙和田园土1∶1混合，成活率高达86．0％。这为厚朴的组培工厂化育苗提供了技术理论依据。%By orthogonal test and single factor randomized block design ,the best medium combinations for the tissue culture of Magnolia seedlings were investigated inclucling those for the stage of bud inducing cul -ture,proliferation culture,rooting,seedling cultivation and transplanting hardening by means of range analysis , variance analysis and LSD multiple comparison .The test showed:the combination of B 5 ,6-BA 5.0 mg/L,NAA 1.0 mg/L+2,4-D 1.0 mg/L was the best for bud induction;the combination of B 5 ,6-BA 5.0 mg/L+NAA 0.1 mg/L+sucrose 15 g/L was the best for Proliferation culture;shoots grew robustly in the medium combina-tion of B5+NAA 0.5 mg/L+GA3 0.3 mg/L;besides,the rooting rate was the higest-62.7%in the medium of B5+IBA 1.0 mg/L+NAA 1.0 mg/L;ABT1 & ABT3 could also induce rooting ,but the rooting rate was in the combination of B 5+ABT3 1.5 mg/L+ucrose 20 g/L was 1.5%less than the higest .The transplanting survival rate was 92.2%when the seedlings were treated with closed bottle hardening 6 days and corkage 6 days in nat-ural light;the survival rate was up to 86%when tansplanting matrix was a 1 ∶1 mixture of sand and garden soil.The study will provide technical and theoretical basis for its tissue culture of seedlings .