Multiple propagation methods of Moringa Oleifera in vitro plantlets using callus culture

摘要

The present invention relates to a method of forming in vitro plantlets of moringa. The method comprises: a step of forming a callus and inducing multiple shoots in growth medium, and redifferentiating plantlets formed; a step of growing the redifferentiated plantlets; and a step of acclimatizing the plantlets, wherein the step of growing plantlets comprises growing plantlets that have been cultured during the step of forming plantlets and redifferentiating plant bodies, in a medium prepared by adding, to a basal medium, 30-40 g/L of sucrose, 30g/L of D-sorbitol, 0.1 g/L of inositol, and 8-10 g/L of agar. Accordingly, the method of the present invention is advantageous in increasing the number of plantlets by increasing biological activities without causing abscission of leaves in a proliferating culture process that expands the culture scale, and can shorten the total plantlet formation period to 3-4 months, thereby enabling the supply of moringa in a large quantity to farms. Further, even in the Korean climate which permits a rather short cultivation period from May to October, the method of the present invention allows harvesting of leaves, stems, and roots in the farms, which leads to increased income of the farms and serves to replace imported moringa.