烟酸诺氟沙星在松浦镜鲤体内代谢残留规律的研究

摘要

分析烟酸诺氟沙星在松浦镜鲤体内代谢残留规律，准确制定该种药物的休药期，为有效、安全、合理地使用烟酸诺氟沙星提供准确科学的理论依据。选用松浦镜鲤（ Cyprinus carpio L．）为实验对象，连续5 d 口灌30 mg／（kg·d）烟酸诺氟沙星（norfloxacin nicotinate，NFX）后，分别于给药后1，2，3，4，5，6，7，9，11，13，15，20，25 d采集鲤血浆、肌肉、皮、肝和肾5种组织，通过高效液相色谱法检测其各组织内烟酸诺氟沙星的残留量。烟酸诺氟沙星在鲤体内吸收分布迅速，代谢较为缓慢，各组织分布量由多到少依次为：肾脏、肝胰脏、肌肉、皮肤、血浆，为避免组织内残留过多药物，建议休药期为24 d。%The aim of this study is to study the residual rule of norfloxacin nicotinate in common carp,for⁃mulate the withdrawal time of norfloxacin accurately and provide a precision and scientific theory basis for u⁃sing this medicine reasonably,safely and effectively.In the experiment,common carp(Cyprinus carpio L.)was taken as the target,the fish were drenched with the medicine 30 mg/( kg ·d) for 5 days,then their kidney,liv⁃er,muscle,skin and blood were collected at the 1st,2nd,3rd,4th,5th,6th,7th,9th,11th,13th,15th,20th,25thday af⁃ter the medication.The residual of norfloxacin nicotinate in the above 5 kinds of tissues was detected by high performance liquid chromatography(HPLC).Norfloxacin nicotinate was absorbed by the tissues rapidly but me⁃tabolized slowly.The distribution of the medicine was in the order:kidney>liver>muscle>skin>blood.In order to avoid excessive medicine residues within the tissues,the 24 days of withdrawal time is proposed.