矾根的组织培养与快速繁殖

摘要

以矾根带茎尖幼嫩茎段为外植体,以MS为基本培养基,研究了不同激素配比条件下矾根的组织培养与快速繁殖技术.结果表明:适宜的诱导增殖培养基为MS+ 6-BA 0.5 mg/L+ NAA 0.05 mg/L;继代培养基为MS+6-BA0.1 mg/L+ NAA0.01 mg/L;生根培养基为1/2 MS+ NAA0.2 mg/L+ AC 1.0 g/L.生根苗移入泥炭土:珍珠岩=2:1的基质中,成活率达80％以上.%The young stem segments with stem apexes were used as explants,and MS as minimal medium to study the technique of tissue culture and rapid propagation of H. Micrantha under different hormone concentrations. The results showed that the appropriate media were MS +0.5 mg/L 6-BA + 0.05 mg/L NAA for induction and proliferation,MS + 0.1 mg/L 6-BA + 0. 01 mg/L NAA for subculture, and 1/2 MS+ 0.2 mg/L NAA+ 1.0 g/L AC for rooting. The rooted plantlets were transplanted into the substrate(peat soil:perlite = 2:1) and their survival rate was over 80%.