携带四环素耐药基因的鸭源大肠杆菌质粒不相容群分析

摘要

目的:用PCR方法测定22株携带四环素耐药基因鸭大肠杆菌所含质粒的不相容群.方法:设计18对引物,分为5个多重PCR体系和3个单一PCR体系,用来检测识别FIA、FIB、FIC、HI1、HI2、I1、L/M、N、P、W、T、A/C、K、B/O、X、Y、F和FⅡ复制子.结果:22株鸭大肠杆菌中,分别有7、4、9、2、6、2、3、7株和19株属于IncI1、IncN、IncFIB、IncY、IncP、IncFIC、IncA/C、IncK和IncFⅡ.试验菌株所含质粒大部分属于IncFⅡ,占86.4％,属于1、2、3、4、5个不相容群组合的分别为13.6％、31.8％、31.8％、18.2％和4.5％.%Objective: The incompatible groups of the plasmids carrying tetracycline resistant genes in 22 duck Escherichia coli I-solates were determined by using PCR method. Methods; 18 pairs of designed primers were divided into five multiple PCR systems and three single PCR systems, and they were used to detect FIA, FIB, FIC, H11, H12, II, L/M, N, P, W, T, A/C, K, B/0, X, Y, F and FⅡ replicons. Results; seven, four, nine, two, six, two, three, seven and nineteen of 22 E. Coli isolates from duck belonged to Incll, IncN, IncFIB, IncY, IncP, IncFIC, IncA/C, IncK and IncFH, respectively. Most (86.4%) of plasmids in the tested strains belonged to IncF11. One incompatible group and 2,3,4 and 5 incompatible group combinations accounted for 13. 6% , 31. 8% , 31.8% , 18.2% and 4.5% , respectively.