一种发根农杆菌介导的大豆“组合”植株的高效创建方法与利用

摘要

利用微型注射器注射含发根性Ri质粒载体的发根农杆菌K599菌液于大豆幼苗子叶节部位，然后在光照培养箱中培养，发根率高达90％，PCR检测阳性率高达100％；随后剪掉原有的根系，在营养液中培养成根部足够健壮的大豆“组合”植株；初步分析了大豆一个ERF蛋白基因的抗、耐旱功能。本方法快速、简单、高效，为后续的大豆功能基因组学研究提供了重要的实验体系。%The cotyledonary node of soybean seedlings was injected by the solution of Agrobacterium rhizogenes strain K599 with rooting Ri plasmid vector through using micro -syringe, then they were cultured in illumination incubator .Their rooting ratio was up to 90%, and the positive ratio reached 100% by PCR detection.Hereafter, their original root system was cut off , and they were cultivated to become composite soybean plants with strong enough roots in nutrient solution .The drought-resistant and drought -tolerant functions of ERF protein gene in soybean were primitively analyzed .This method was fast, simple and highly effective, and it provided an important experimental system for subsequent soybean functional genomics studies .