基于白木香 ESTs的沉香属 SSR分子标记引物筛选

摘要

A large amount of Aquilaria sinensis ( Lour.) Gilg EST sequences were obtained through high -throughput sequen-cing by 454GS FLX Titanium.From these EST sequences, 956 SSRs were selected.Forty-four pairs of EST-SSR primers were designed to amplify flanking SSRs from 956 selected SSR-ESTs.Twenty-two pairs of primers were confirmed to be efficient in A. sinensis.The preliminary validation of the polymorphism of these 22 pairs of primers was further carried out in 9 samples of 3 species in Aquilaria, and the results showed that all these primers could be used in Aquilaria.This work lays a foundation for the consequent family analysis of A.sinensis and genetic diversity analysis of plants in Aquilaria.%通过对白木香样品的454GS FLX Titanium高通量测序，获取了大量白木香EST序列，从中搜索出956个SSR，根据引物设计标准，设计了44对EST-SSR引物。在合适的PCR反应体系下，以海南白木香样品的DNA为模板，对设计的EST-SSR引物进行筛选，发现有22对EST-SSR引物能较好地扩增出产物。在沉香属3个种9份样品中进一步对这些可扩增的引物进行多态性初步验证，所筛选引物均可在所有沉香属样品中使用，为后续白木香居群及沉香属植物遗传多样性分析奠定基础。