宁玉草莓茎尖组培快繁技术研究

摘要

The stolons of strawberry variety “Ningyu” were used as explants .After disinfection , we studied and selected the appropriate culture media for stem -tip differentiation, subcultural propagation and rooting .The results showed that the best method of disinfection was using 75%ethanol for 40 s plus 0.1%HgCl2 for 6~8 min;the best differentiation medium was MS +0.5 mg/L 6-BA+0.1 mg/L NAA;the best medium for subcultural propagation was MS +0.5 mg/L 6-BA+0.1 mg/L NAA, and its multiplication coefficient was 9;the best rooting medium was 1/2MS+0.05 mg/L NAA, and the rooting rate reached 96.6%.%以宁玉草莓匍匐茎为外植体，进行消毒灭菌、茎尖分化、继代增殖和生根培养基筛选研究，结果表明：最佳消毒方法为75％乙醇40 s＋0．1％升汞6～8 min；草莓茎尖分化培养基：MS＋6-BA 0．5 mg/L＋NAA 0．1 mg/L；继代增殖培养基：MS＋6-BA 0．5 mg/L＋NAA 0．1 mg/L，其增殖系数为9；生根培养基：1/2MS＋NAA 0．05 mg/L，生根率可达96．6％。