利用种子性状QTL定位高油玉米淀粉含量QTL

摘要

F2.3 populations consisted of 284 plants were developed from the cross between a normal maize inbred 8984 and a high-oil maize inbred GY220,and a genetic linkage map including 185 SSR markers constructed by (8984/GY220) F2 population. The quantitative trait loci (QTL) of starch content were identified and their effects were evaluated using SeedTraitQTL containing maternal effect. A total of seven QTL were detected in the F;., population,located on chromosome 5,8 and 10 respectively. Six QTLs showed partially dominant effects but qSTA8-3 showed additive. Contribution of single QTL to phenotypic variation varied from 5.87% to 10.93% ,and total contributions of all QTLs were 53.38% . The allele of all QTLs were contributed by the normal maize parent 8984. The QTL qSTA5-2 with higher contribution could be used as the main objective QTL in fine mapping.%以普通玉米自交系8984与高油玉米自交系GY220为亲本组配得到284个F2:3家系群体,利用185个SSR标记构建玉米遗传连锁图谱.通过包含母体效应的种子性状QTL作图方法对玉米籽粒淀粉含量进行QTL定位和效应分析,共检测到7个与淀粉含量相关的QTL,分别位于第5,8,10染色体上,除qSTA8-3的遗传作用方式表现为加性外,其余QTL作用方式为部分显性.单个QTL贡献率为5.87％～ 10.93％,累计贡献率为53.37％.所有QTL的增效基因均来自普通玉米亲本8984.淀粉含量QTL qSTA5-2贡献率较大,可以作为进一步精细定位的主要目标QTL.